Simultaneous measurement of dissolved oxygen concentration and velocity field in microfluidics using oxygen-sensitive particles

This paper reports a technique for measuring the velocity and dissolved oxygen concentration (DOC) fields simultaneously in a micro-scale water flow using oxygensensitive particles (OSP) and a conventional microparticle image velocimetry method. The OSP were fabricated using a dispersion polymerization method by synthesizing platinum (II) octaethyporphyrin (PtOEP) with polystyrene, and used as tracer particles and oxygen sensors. An ultraviolet lightemitting diode with a wavelength of 385 nm was used as the excitation light source, and phosphorescence images of OSP were captured on a CMOS high-speed camera. The interrogation window concept was used to measure the DOC in water from the dispersed phosphorescence intensity distribution of OSP. The Stern–Volmer equations in the interrogation windows were obtained from in situ calibration. Water containing OSP with DOC values of 0 and 100 % were injected into a Y-shaped microchannel using a double loading syringe pump. The velocity and DOC field over the entire channel area were quantified.